The Langley Nicotine test is used to determine the relay-site of autonomic fibers. This test stimulates the preganglionic fiber and notices the response that appears. By adding a large dose of nicotine, the ganglion (site of relay between pre- and postganglionic fibers) is blocked. Now the preganglionic fiber is restimulated again. If there is still a response, the fiber does not relay in the ganglion. But if there is no response, the fiber relays in the ganglion.
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