What is ELISA? How it is useful in CRP Level measurements?
ELISA is enzyme-linked immunosorbent assay. It is an immunological procedure and hs-CRP test is based on the principle of a solid phase ELISA. It utilizes a unique monoclonal antibody directed against a distinct antigen determent on the CRP molecule. Solid phase antibody used is frequently mouse monoclonal anti-CRP immunoglobulin. Test sample is set and allowed to react simultaneously with two antibodies resulting CRP to sandwich between solid phase and the enzyme-linked antibodies. After some time of incubation, unbound antibodies are removed and the bound antibodies are color labeled. The labeled antibodies are than observed by spectrophotometers at 450nm, & the intensity of the light emitted from the assay is taken directly proportional to the CRP concentration in the sample.
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ELISA CRP Testing Back To Heart FAQ
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