Most experts and healthcare professionals agree that Smoking, and thus, nicotine is unquestionably the most addictive thing in use today. The principle source of nicotine is tobacco products. Tobacco smoking or chewing results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into 20 metabolites excreted in urine including the primary metabolite Cotinine. Cotinine metabolite is the primary marker for nicotine tests and tobacco use screening.